type strain c jejuni atcc 33560  (ATCC)

Journal: bioRxiv

Article Title: Dual species interactions shield Campylobacter against multiple antibiotics

doi: 10.64898/2026.02.27.708565

Figure Lengend Snippet: Resistance patterns of MC14-02991.B and derivatives Cj14-02991.8 and Efs22-05285.1. MIC values above the ECOFF for mixed samples and purified strains are marked bold and the respective isolates are categorized as non-wildtype for this antimicrobial. If a MIC value is below the ECOFF, the corresponding isolate is categorized as wildtype for this antimicrobial. Since there is no C. jejuni ECOFF for meropenem available from EUCAST, ertapenem ECOFF is given instead. Currently, no Enterococcus ECOFFS or breakpoints are available for azithromycin, clindamycin and nourseothricin (EUCAST) (see Material and Methods) and therefore the respective MIC values cannot be categorized into wildtype/ non-wildtype. The ASA cluster is underlined. See material and methods for full panel.

Article Snippet: To assess whether this is a more general feature of C. jejuni / E. faecium interaction, we applied this assay on the type strain C. jejuni ATCC 33560 with and without co-incubation with MDR E. faecium strain EFm22-05284.1.

Techniques: Purification

Journal: bioRxiv

Article Title: Dual species interactions shield Campylobacter against multiple antibiotics

doi: 10.64898/2026.02.27.708565

Figure Lengend Snippet: : Images from two colonies each of mixed samples MC14-02991.B and MC20-00984.21 are shown. Bacteria were grown on Brucella agar or CCDA. For sample MC14-02991.B, coccoid bacteria were found in all investigated colonies of the mixed cultures. The cocci were either intermingled with Campylobacter , as shown here, or present in smaller groups. For MC20-00984.21, coccoid bacteria were found in some but not in all investigated colonies of the mixed samples. They were always intermingled with the spiral shaped Campylobacter and never appeared in groups. : Images from colonies of separated strains. From left to right: C. jejuni (Cj14-02991.8), E. faecalis (Efs22-05285.1), C. jejuni (Cj-20-00984.4) and E. faecium (Efm22-05284.1). Bacteria were grown on Brucella Agar or CCDA. On Brucella agar, C. jejuni may develop an unusual round to pleomorphic morphology (arrow) which can be the dominant form (see also , Cj ATCC 33560). : Images from colonies of reference isolates. From left to right: C. jejuni (Cj ATCC 33560) grown on CCDA, C. jejuni (Cj ATCC 33560) grown on Brucella agar, E. faecalis (Efs ATCC 29212) grown on Brucella agar, E. faecium (Efm ATCC 19434) grown on CCDA.

Article Snippet: To assess whether this is a more general feature of C. jejuni / E. faecium interaction, we applied this assay on the type strain C. jejuni ATCC 33560 with and without co-incubation with MDR E. faecium strain EFm22-05284.1.

Techniques: Bacteria

Journal: bioRxiv

Article Title: Dual species interactions shield Campylobacter against multiple antibiotics

doi: 10.64898/2026.02.27.708565

Figure Lengend Snippet: First a microdilution assay was performed for every test antimicrobial for 24h incubation at 42°C (microaerophilic). For re-isolation of C. jejuni , 5 µl suspension from each well was transferred to Brucella agar containing Campylobacter selective supplement and incubated at 42°C for 48h under microaerophilic conditions. The agar did not allow growth of E. faecalis or sufficient growth of E. faecium within the given time of 48h. The isolation MIC is the lowest concentration of the antimicrobial, where no C. jejuni could be isolated from the wells, i.e. no growth was visible on the agar plate. The test was used for the following antimicrobials: azithromycin, clindamycin, tetracycline, gentamicin, meropenem and nourseothricin.

Article Snippet: To assess whether this is a more general feature of C. jejuni / E. faecium interaction, we applied this assay on the type strain C. jejuni ATCC 33560 with and without co-incubation with MDR E. faecium strain EFm22-05284.1.

Techniques: Microdilution Assay, Incubation, Isolation, Suspension, Concentration Assay